[Wed Aug 24 18:10:04 2016] >Input file: TM2A65ATD.pdb >TM segments: A20-32_A43-59_A89-126_A166-179_A201-216_A244-261_A283-298_A335-360_A381-394_A403-422_A450-465_A487-500 MAIDEN score = -161.2506 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- K121A-L126A 10 I106A-V386A 1.26586596768758 V210A-I297A 1.20915666882535 I100A-A407A 1.18201768749181 I100A-F259A 1.1791688361135 P101A-I359A 1.168294705319 I106A-A390A 1.12611990416096 F122A-F342A 1.06061331709837 E287A-I292A 1.05475677203122 W99A-F494A 1.02750892848516 V95A-F494A 0.980295834961921 E112A-L348A 0.950343769539826 F252A-V413A 0.928456878549601 Y47A-I251A 0.907309637285262 F252A-L257A 0.900434057509676 F259A-V412A 0.894195212872352 L205A-L347A 0.876543051169916 F252A-V412A 0.844500869208482 Y450A-I455A 0.844331303186643 L116A-F349A 0.831710917937603 V103A-D404A 0.812237960775949 L209A-I297A 0.811525680459156 I245A-L463A 0.81076997847098 W114A-F172A 0.809334532168526 L25A-L348A 0.803670260047505 V213A-I297A 0.784477179301946 L102A-I359A 0.783408732333824 P101A-A261A 0.782464121633624 L417A-L460A 0.769360090486949 L205A-W344A 0.756684309373435 V207A-L293A 0.744012566211121 L257A-V412A 0.737956675941898 F51A-I56A 0.735480403267568 L202A-L347A 0.731545730695744 A119A-W344A 0.722755470086761 T115A-L348A 0.722752938917589 L205A-L343A 0.722531667523639 L123A-F339A 0.716073785253303 V23A-T254A 0.715752904593182 W114A-A168A 0.7082405489038 F122A-F339A 0.701807154614186 L53A-V451A 0.69804471533106 F43A-A247A 0.670384974709123 I100A-G411A 0.669394530502346 Y110A-A390A 0.668420132117797 G249A-V413A 0.665520846626091 T115A-F346A 0.660656115818361 M176A-A383A 0.659214461327788 V23A-E290A 0.654799830383561 N21A-A261A 0.646196081950064 L53A-T456A 0.643022101777685 W99A-A407A 0.642467624107982 F43A-W244A 0.632029281707864 F28A-G295A 0.624024228358284 L52A-P57A 0.623481071323556 G117A-F167A 0.621109737407282 F253A-G408A 0.617437501272309 W114A-V171A 0.617243149218682 F31A-G294A 0.616738945111888 F31A-G295A 0.616730557775734 S113A-F172A 0.612381568122524 L25A-E112A 0.612048140962152 L420A-R453A 0.607028927280436 F253A-T409A 0.600697839714284 A206A-L347A 0.595238762834768 V413A-L460A 0.592211902581763 I100A-G408A 0.591531311579605 M59A-A283A 0.590636267892294 L417A-A461A 0.586977144541013 P32A-G294A 0.586766762483447 A119A-L343A 0.577752424599014 V210A-L293A 0.577411702525813 Y107A-D404A 0.5767638631979 I100A-V412A 0.575100403168612 V109A-G352A 0.570158179313854 F259A-I359A 0.569751467526307 V103A-I490A 0.56546263178729 I120A-F342A 0.560964664211737 I56A-A283A 0.55908599031854 F96A-F415A 0.558310035417725 P101A-F259A 0.557885368655136 L58A-L257A 0.548991894016737 A206A-W344A 0.54642102860935 L116A-V171A 0.542340222324015 F31A-E290A 0.540493251186169 L202A-A351A 0.532495864481547 F118A-F345A 0.526493344525443 Y110A-V393A 0.519995786486917 S113A-I174A 0.51990461305572 F118A-F342A 0.516924227365871 N27A-V291A 0.516841698384304 F28A-L293A 0.516816796190867 N21A-I359A 0.510415161977234 I106A-F387A 0.503231359865644 V95A-L497A 0.500632676072411 M360A-T382A 0.496262478129403 F28A-E290A 0.496250747712885 F414A-L464A 0.491743275704026 E290A-G295A 0.490287792935067 G97A-F415A 0.48109512867439 Q250A-L255A 0.47738323537482 P101A-G260A 0.47671018654306 S113A-F349A 0.471625723326242 L202A-F350A 0.460851710412912 L53A-I455A 0.456812628621718 P57A-E419A 0.455212569461673 A22A-L255A 0.454654746745602 A105A-V386A 0.453958486955872 I421A-P457A 0.453708553507168 N21A-I357A 0.45057168800717 G260A-I359A 0.450200079657811 L123A-F342A 0.44821404033873 F28A-L348A 0.44549233321812 V23A-A289A 0.444682394914402 F28A-I297A 0.444079764698883 G24A-T254A 0.444057730275749 M59A-L255A 0.443289709237299 F405A-I410A 0.44021240917751 I100A-G260A 0.425153443664724 L420A-M452A 0.423842445300945 L116A-F345A 0.423559435331611 L116A-F346A 0.42273112582438 L420A-P457A 0.419525083712696 L102A-V386A 0.419475735923948 V109A-T175A 0.415371056057336 V109A-L353A 0.413423211017059 E419A-T456A 0.410338422328493 V109A-F172A 0.409931336421971 K288A-L293A 0.408000403723682 F252A-G258A 0.407957633286658 Y47A-W244A 0.392103752573013 K121A-F342A 0.385991412810372 L102A-M360A 0.384977033108794 A105A-I359A 0.381673040075338 A351A-A358A 0.379181816424825 V291A-S296A 0.378071312179581 L209A-W344A 0.377413498913473 A119A-F349A 0.37261887234439 F203A-A289A 0.366426932865664 L417A-P457A 0.363223777367697 V213A-S296A 0.363193416028828 G26A-Q250A 0.362714172203767 F96A-F494A 0.358988276861293 L255A-A283A 0.356409028639415 G55A-L255A 0.352875723409652 Y47A-G295A 0.350755800517131 I100A-M403A 0.350589457245546 G55A-F252A 0.346793703838038 A105A-M360A 0.346230538929092 L58A-M452A 0.344200279906933 G24A-E287A 0.344018357889406 V23A-Y108A 0.343151734001631 F51A-I251A 0.343112583235382 I92A-T498A 0.341230888048806 G26A-E112A 0.340222834317109 V54A-V248A 0.340020043556083 F259A-F415A 0.33640517808103 I106A-V393A 0.336286906673064 L116A-T175A 0.333054917650054 L98A-F259A 0.330592671565084 S113A-A168A 0.326678850820232 P101A-A407A 0.32315468723943 V23A-L255A 0.321523228045193 L173A-F387A 0.321366555921301 G260A-A407A 0.321193036174296 L98A-L497A 0.319712937827748 L255A-N286A 0.319690529445527 P101A-W406A 0.319430918222533 G249A-A407A 0.318800529011016 M44A-A247A 0.318407307123933 F253A-V412A 0.315854190058685 T254A-T354A 0.315798490753299 M403A-R487A 0.31499621952626 S113A-A390A 0.313318877220636 L58A-L420A 0.313252421145193 V104A-Y489A 0.312761382552751 Y108A-D404A 0.311358900074171 V23A-L285A 0.310323780539203 L93A-T418A 0.310242292655078 M403A-G408A 0.309291475106451 A22A-G55A 0.308925792656832 G24A-L348A 0.306876294723442 L123A-T338A 0.306829309576941 V109A-M176A 0.305962419427495 L58A-G416A 0.304640988877286 P46A-V248A 0.303692171674246 V103A-W406A 0.30293146307436 F414A-L460A 0.302575541157214 V207A-R212A 0.301723515631372 G20A-A261A 0.301656063671762 V104A-I359A 0.300558130111662 Y107A-V393A 0.299573172389543 V109A-S356A 0.296199441878646 P57A-T284A 0.295406407159424 P57A-V451A 0.294177840799971 W99A-L493A 0.293380744492845 M452A-P457A 0.292979278732731 A50A-A247A 0.292601185081255 D404A-T409A 0.292296366417617 E287A-L293A 0.291416517789685 A50A-I251A 0.291161542021603 W99A-L497A 0.290523211692988 E112A-F177A 0.29051484765314 L209A-L340A 0.287445184571542 G24A-T354A 0.287276482592572 G55A-L460A 0.287147363548169 M59A-L257A 0.287064872080881 M44A-Q250A 0.285685037667708 L202A-G294A 0.283546094870294 L255A-G260A 0.283136899834999 R212A-L340A 0.281266826323282 F122A-G341A 0.280702084076878 G26A-F253A 0.280502873769874 N27A-P32A 0.279234990987742 F51A-V248A 0.279192501873515 S389A-G492A 0.278981536090728 A22A-A283A 0.278142558600354 G125A-L340A 0.27728389936169 L98A-G260A 0.276113466911704 F28A-S298A 0.275758987050894 L205A-G341A 0.274296525789023 A50A-W244A 0.272834558433384 L93A-G411A 0.272607238283834 L93A-E419A 0.271022617024759 V413A-M452A 0.266373105682067 V23A-E287A 0.264519294633415 I106A-M360A 0.264125469958504 A335A-G341A 0.263558837735069 M403A-I490A 0.263459871275046 Y47A-Q250A 0.262030749117002 L93A-G416A 0.261976723014614 R30A-E290A 0.261070700376161 V413A-L464A 0.259990576683281 S113A-N179A 0.259517601297097 R30A-A247A 0.258760157610523 L173A-H391A 0.258724193594834 L205A-G294A 0.2557184154108 F28A-G294A 0.249695093611427 Y47A-A247A 0.248920574555572 L209A-G337A 0.248855215917518 L58A-G260A 0.248596964484072 A261A-I359A 0.248469088380536 G24A-Q250A 0.246920873642376 V104A-W406A 0.246879189887625 Y107A-M403A 0.246365957851679 G26A-S356A 0.245899429761149 F31A-Q250A 0.245613561126001 G94A-G411A 0.244625788086967 G55A-I251A 0.244448285961605 L98A-G411A 0.243407695085208 T115A-F345A 0.242937572322926 Q250A-E287A 0.242705187301585 G94A-F259A 0.241747509324283 A90A-G97A 0.241603411194079 M176A-F387A 0.240105962066033 V54A-I251A 0.239730065156956 T201A-L348A 0.239657066058497 L53A-M452A 0.238418755358702 T254A-L285A 0.237205408796541 A390A-Y489A 0.236967328933272 Y108A-H391A 0.236591774478191 F405A-G411A 0.234946563187442 Y110A-F172A 0.234351324380614 N27A-S296A 0.233338998844069 S113A-A351A 0.231384795109244 G416A-F459A 0.230926515522813 N27A-G249A 0.230499841683967 N21A-G258A 0.230014668992824 N27A-S298A 0.228907096018766 F253A-G416A 0.227571807940782 L257A-G416A 0.225778973154783 G258A-F414A 0.225778229969237 V23A-S355A 0.22564741143587 G20A-I359A 0.221857407514038 S356A-A383A 0.221379434546028 A206A-S298A 0.220262005451927 L209A-G295A 0.219557576534495 Y108A-A358A 0.219355598834936 L98A-T382A 0.21930385822041 V213A-G295A 0.219252623147008 V104A-G258A 0.218959629650084 N21A-P101A 0.218151363527982 G55A-L257A 0.217421030681019 F96A-A261A 0.217264699075701 G97A-V104A 0.217225387081031 G20A-S355A 0.215689021912825 G24A-V109A 0.21478220920442 N27A-Q250A 0.214102962416633 M59A-V451A 0.213624579285092 Y110A-M394A 0.21360362286916 M59A-V412A 0.212524375176002 V386A-M403A 0.211737329067208 F214A-I297A 0.211274453207627 F172A-V386A 0.210801527184984 I251A-S256A 0.210715702314204 V248A-G416A 0.210057853081503 V23A-L202A 0.209461183850352 G20A-A351A 0.209298756178191 V291A-S298A 0.208254289757665 I204A-W344A 0.206034977223046 Y107A-E112A 0.205621493476386 V95A-F259A 0.205565622086145 T115A-I178A 0.205453723727305 I120A-W344A 0.204915806523875 M59A-V413A 0.20413317718545 G55A-V451A 0.203614622391176 Q250A-F405A 0.203392083775292 V95A-T498A 0.203029534053488 T418A-P457A 0.202505371382797 I111A-T354A 0.202495786247298 V23A-T354A 0.201837624295036 A119A-F345A 0.201552939534665 A206A-L293A 0.200734028501362 V54A-R453A 0.20026537691965 V23A-F31A 0.200224442833916 R30A-V291A 0.199681579827431 F414A-V462A 0.199496095278286 W114A-F349A 0.199206176468499 V109A-F177A 0.199178086035437 D404A-R487A 0.197687430869847 F96A-G408A 0.197413750901521 V103A-G492A 0.197156902298618 G294A-W344A 0.196844942305113 V103A-S356A 0.195477578634772 N21A-L255A 0.195447093800237 I251A-V291A 0.195038824836255 L29A-G294A 0.194273250480028 I100A-G258A 0.194051902001753 T175A-A384A 0.19176812068913 V103A-H391A 0.191709439069386 L29A-T115A 0.189843096445565 N286A-G295A 0.189692574106253 I422A-T456A 0.189540487318484 F28A-E112A 0.188926346769764 N21A-L353A 0.188493915146549 V23A-R30A 0.187991541877346 Y108A-S256A 0.18770701317565 Y110A-S355A 0.187003090298286 F405A-V413A 0.186596859782929 V103A-M403A 0.186368769357309 Y110A-M360A 0.185426249468435 G20A-G260A 0.18376350079809 N179A-L353A 0.18356339502111 V95A-L495A 0.183302802892934 I410A-L464A 0.183047888646168 R212A-W344A 0.182615446138977 V393A-I490A 0.182450233894217 Y107A-S355A 0.182305353523565 G117A-G125A 0.181869953459263 N27A-L293A 0.181386583183553 G24A-L353A 0.181222219509587 T175A-A351A 0.179966367784744 R212A-A335A 0.179822107061416 G249A-L417A 0.179176601549165 Y108A-F350A 0.178138365758459 G26A-Y107A 0.177786685210345 T201A-A289A 0.177335940046048 F31A-K288A 0.17690381536052 F51A-K288A 0.176624272023816 K121A-L340A 0.17590089105013 F252A-G260A 0.175833031915316 L116A-K121A 0.175531982118606 G117A-I170A 0.175222488678905 V109A-M403A 0.17506103709645 P101A-G352A 0.17439375035799 Q250A-G258A 0.17395272286883 A105A-S113A 0.173785240347261 I49A-G55A 0.172729217227602 S113A-V171A 0.172560416490827 S298A-L340A 0.172520559980154 S298A-L347A 0.172417163574732 S113A-L166A 0.172126238254127 A22A-F28A 0.172071686701795 F167A-H391A 0.171607584733976 F43A-A246A 0.171238376523817 G117A-G352A 0.170574630825756 F31A-V291A 0.170049301459433 T254A-A358A 0.169833347424015 I251A-L257A 0.169818444568676 F51A-E290A 0.169388481136276 N21A-F349A 0.168738192682336 L52A-V451A 0.168536721998876 Y110A-T175A 0.168519209950457 G97A-F259A 0.16850295120886 T201A-W344A 0.168465609588536 P32A-S298A 0.167924111528147 L209A-L343A 0.167277208631995 W244A-L463A 0.167082507374662 E112A-Y169A 0.166621645352991 G258A-V412A 0.166483186013217 A289A-I297A 0.165995530503388 I100A-G416A 0.16585983925409 M403A-G492A 0.165798054691882 L52A-T284A 0.165529542690358 Y108A-A351A 0.165221912616944 T201A-F349A 0.165002845438523 V54A-G258A 0.164447993402234 W381A-L500A 0.163190328192908 G24A-A206A 0.163122044038309 W99A-T382A 0.162995246604035 L25A-S355A 0.162572548509248 G295A-A351A 0.162511666353303 G94A-G258A 0.162329260403241 L58A-T418A 0.162251694014664 G55A-L285A 0.162210224204717 F252A-A407A 0.161761055990811 A283A-G294A 0.161636831452177 A247A-G416A 0.161636831452177 I111A-I174A 0.161533570023484 A289A-G294A 0.161416341233293 G260A-G411A 0.161131726989783 V104A-M403A 0.16108122593374 E112A-H391A 0.160787690929356 W99A-G258A 0.160772046919392 G26A-A407A 0.160684955449709 M44A-G249A 0.160086933103298 S113A-K121A 0.159509954116547 I48A-I245A 0.159314933691918 G294A-A351A 0.159282598342218 E112A-L353A 0.159198522325037 L126A-G341A 0.159083580067456 G26A-G408A 0.157979535233501 G125A-T338A 0.157901791510472 G117A-L348A 0.157875494269813 M176A-V393A 0.157839980362542 F203A-S296A 0.157724279596196 F405A-F415A 0.157531433861494 L126A-G337A 0.15729288386415 G26A-G295A 0.157181717293791 T115A-T354A 0.15679988219116 Y107A-W114A 0.156262473118878 N21A-L202A 0.15582436524747 I92A-I100A 0.155742764989426 G249A-G416A 0.155205749578317 A22A-I357A 0.15447705320615 I106A-I111A 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