[Thu Aug 25 00:29:32 2016] >Input file: TM2B2FATG.pdb >TM segments: A13-26_A42-58_A94-106_A117-132_A152-167_A191-204_A223-236_A249-262_A272-286_A300-315_A344-360 MAIDEN score = -72.6935 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- G161A-W302A 10 H157A-H305A 3.82931286371974 W198A-I254A 1.24511828234097 I158A-L310A 1.23585781810656 W234A-I281A 1.2275603618181 V20A-V99A 1.18832852568548 I158A-W302A 1.17291442732321 I260A-I275A 1.15615320245022 M19A-L197A 1.11482503976407 I275A-W311A 1.08789480979667 A255A-I304A 1.08732369594747 D300A-H305A 1.04117293176102 W234A-I249A 1.02306448455289 W54A-M94A 0.978193131783009 V229A-I273A 0.976961853469406 V23A-I103A 0.964754595554576 F96A-V156A 0.953769838598668 C283A-I304A 0.94944544039676 V20A-F44A 0.931684582815833 F162A-F353A 0.921099309933152 I158A-H305A 0.8695666676171 V16A-F96A 0.847971750013125 W201A-L257A 0.824378624126989 M235A-I249A 0.822303069147927 F132A-T348A 0.811569211043626 I158A-I307A 0.792281209246795 L165A-W302A 0.779491461299735 T223A-L257A 0.761764304311475 L104A-S160A 0.736042761402788 S46A-I118A 0.731168842170864 I158A-T348A 0.730713691611169 V16A-W201A 0.717771428149633 V167A-T356A 0.712046868870091 A279A-W311A 0.695563658438355 W198A-L257A 0.681354917829044 V16A-M95A 0.677230130864545 Y351A-T356A 0.674025648145035 L42A-A102A 0.673545319449684 F25A-I192A 0.67183791499737 F26A-I192A 0.669109770005014 L120A-L359A 0.668960394659789 F232A-I249A 0.667275613281165 A259A-W311A 0.666123555505082 L104A-A163A 0.657273742426319 S251A-I304A 0.65267813774298 F132A-V155A 0.645375109247378 V229A-L277A 0.64225668577281 S160A-G306A 0.637283230520545 V233A-L277A 0.635455846162241 S43A-I101A 0.631782937823434 I260A-A272A 0.631299219638312 V229A-G280A 0.625679080019627 V23A-L197A 0.623001004568195 F232A-G252A 0.619577743423946 V16A-F204A 0.611687549824719 F162A-W302A 0.611680581183341 C283A-I307A 0.610717378880221 V20A-L47A 0.604685466777829 M19A-L196A 0.596703972215588 T100A-H157A 0.595508358359531 A163A-W302A 0.59035393731687 F232A-A253A 0.583351209769147 V233A-I281A 0.570324406872851 I275A-A315A 0.569028439837505 A97A-Y129A 0.555718048074376 T50A-M94A 0.555050518949503 P21A-F44A 0.549346266964083 H157A-L310A 0.541320234958933 V128A-Y351A 0.532672357231877 A164A-W302A 0.532279607117616 L15A-F202A 0.529666498731989 S226A-L277A 0.527630927479621 P262A-G309A 0.524515637969856 V23A-A194A 0.522704837656295 C283A-D300A 0.522624277930021 L18A-L196A 0.515498887000065 A286A-I307A 0.511392844592912 L15A-F204A 0.508537938918849 G24A-F44A 0.507567123039099 S46A-S121A 0.506106724507456 L15A-F199A 0.504175296460012 F25A-G193A 0.500619420748095 T223A-A272A 0.500012147736328 W234A-Y284A 0.499665976827271 F162A-T349A 0.498798540639048 F162A-A301A 0.493320035407177 Y129A-V156A 0.492595116704005 S159A-G306A 0.491480082414553 F162A-A352A 0.49110395409433 F26A-L191A 0.484323841177102 L165A-F353A 0.481128363734278 V282A-G308A 0.478179671603021 A230A-V278A 0.477742085818872 V128A-S159A 0.474556342427087 Y284A-A303A 0.473566863237026 V282A-I307A 0.473124454935677 L310A-V345A 0.463590604645958 Y129A-S159A 0.461286931987287 V167A-L357A 0.458438276700569 W234A-A253A 0.456566579650193 A259A-L310A 0.456493812451466 W234A-G252A 0.45543618411029 M154A-S159A 0.451992337448468 V229A-G256A 0.449039365502833 T105A-A164A 0.447402619049426 T261A-G309A 0.445561122895637 T225A-G276A 0.442429706467082 G153A-I260A 0.43591450556687 I254A-I304A 0.434568332069882 A259A-S313A 0.43424318141445 G22A-I192A 0.428195481882481 M17A-M95A 0.416027415739784 V20A-S46A 0.415772482129365 L120A-A360A 0.413892223356649 N224A-L257A 0.412274759077093 G231A-S251A 0.410785945923244 A227A-L257A 0.410205222312831 V51A-A98A 0.407659574911583 S226A-L257A 0.405236615890669 I158A-G308A 0.402898658452395 I103A-G161A 0.401021269286565 W124A-Y129A 0.399412350474722 L53A-A122A 0.398557791581236 G231A-I254A 0.396829629320643 F25A-A194A 0.396719808658476 S159A-T348A 0.396386778248052 I103A-A301A 0.394278936070668 M17A-W198A 0.392076746150885 V229A-A253A 0.390790871487347 W198A-V250A 0.390385405581601 M154A-L310A 0.390288756830643 A97A-V156A 0.389374113172805 A166A-L354A 0.388903008202729 V233A-G252A 0.388159342804518 S159A-T349A 0.387706650440707 I49A-I118A 0.387483585856 W198A-N224A 0.384988863273372 L15A-W201A 0.38310682166329 T13A-M95A 0.382777247801074 Y284A-I304A 0.378736953017498 L123A-V128A 0.378569342584739 L123A-L359A 0.378145312935675 S160A-A301A 0.378094816725254 H305A-S313A 0.377040723560967 A259A-A303A 0.376422633986151 I158A-G306A 0.373887818060056 A163A-L357A 0.373737884772987 L310A-T349A 0.371529902651186 S46A-V99A 0.371374795994107 S160A-W302A 0.36538917591413 V99A-V156A 0.363788605687517 A227A-I254A 0.362256685136271 T50A-A122A 0.359867738179021 G306A-Y351A 0.359109246968169 L42A-T105A 0.357745545148635 V229A-Y284A 0.356990103832224 L104A-A301A 0.35531719089706 W124A-L359A 0.353514288446115 V128A-T356A 0.35240742263883 I260A-S313A 0.351184992044054 G24A-L42A 0.35110290487061 M17A-V99A 0.351062939821494 A228A-A279A 0.348972331410109 L47A-M95A 0.348412416088991 T261A-G312A 0.347944236668918 S226A-A259A 0.34643638005368 V156A-Y351A 0.345031404460494 V278A-W311A 0.34475494827756 S226A-A253A 0.344205403976381 L191A-V250A 0.344036301190108 W302A-I307A 0.342918120412 A97A-L125A 0.339702833860248 I260A-G309A 0.338943223351315 G153A-A259A 0.338191533107143 I260A-G276A 0.336232054965298 A286A-W302A 0.335244937838675 T100A-T126A 0.330670515523404 G306A-A350A 0.328788626977535 T50A-L125A 0.327444689895049 G161A-I307A 0.326805842676196 V314A-V345A 0.325931342827124 G161A-A286A 0.325199289282058 G24A-A98A 0.319000173720257 G22A-A98A 0.317614281053562 S226A-P262A 0.317324751594397 I275A-G312A 0.317081069588008 G161A-A255A 0.313768701671412 L47A-V99A 0.310735620266049 M154A-S313A 0.307320804038336 G161A-A350A 0.306413922722705 L165A-D300A 0.305858399857395 S121A-A164A 0.304867234445842 G161A-I304A 0.304522561780003 A352A-A360A 0.304467692269856 T100A-T105A 0.304152972945728 H157A-C283A 0.304039642199819 G256A-I275A 0.302185389866164 A195A-S251A 0.301445345410955 S159A-G308A 0.299177059108076 I158A-A163A 0.299011162657413 S160A-G308A 0.298156888960407 I304A-G309A 0.297910325217439 G58A-A98A 0.29786520824231 T223A-I260A 0.297626752505474 L165A-A301A 0.297561227735062 G193A-S251A 0.296659314422429 P131A-T356A 0.294321121781424 I103A-S160A 0.293628890224507 S106A-A166A 0.2932083309355 H157A-A258A 0.291839952367616 L285A-A303A 0.291467906388823 A255A-H305A 0.288903057231838 G153A-L310A 0.28698684843935 A164A-D300A 0.286771162314728 L47A-A102A 0.285682416654275 A227A-G308A 0.285093652536356 V128A-V155A 0.284766546770924 M17A-A98A 0.284643014875459 P131A-T349A 0.284424341941578 A279A-I307A 0.284014537773261 S46A-I101A 0.282963266698934 G152A-G203A 0.282274109184772 G24A-L191A 0.282091265941349 F96A-F204A 0.281316886921648 A97A-A122A 0.281298256797749 G22A-L47A 0.281211080862886 V229A-V278A 0.280935299959703 A98A-A122A 0.280917835502399 L196A-G203A 0.279735354702135 G22A-L42A 0.277379250401377 A166A-G306A 0.277149266048278 G200A-N224A 0.276397001398414 S226A-G308A 0.275866821248844 P21A-T50A 0.27577348643467 L47A-A98A 0.275755329052661 W311A-V345A 0.27575525841389 V282A-D300A 0.275696470376869 M154A-P262A 0.274988397487832 M19A-V250A 0.274966498453646 L104A-G161A 0.274654016605354 A227A-I260A 0.273438751031655 C283A-G308A 0.272710171870822 A163A-A303A 0.270734926097838 I45A-I118A 0.270449511655674 W201A-T225A 0.270326675190346 Y129A-G153A 0.268772250927242 I49A-S121A 0.266677516342371 G231A-C283A 0.266376908465627 S160A-A258A 0.266294672349742 A166A-A301A 0.26410051864456 A259A-G276A 0.263258005329711 M154A-G309A 0.262245339616053 A122A-A360A 0.262161031815914 S226A-V274A 0.260350772700947 G252A-L285A 0.260023884027253 W201A-G256A 0.259842788556724 S251A-G308A 0.258818167106643 F26A-A194A 0.254344165990076 A166A-A360A 0.252683548742564 G231A-G308A 0.251859456850561 G153A-F204A 0.251455385773324 M154A-T348A 0.249348074041044 S43A-T105A 0.247860859128 A259A-I275A 0.247846547210397 I101A-L125A 0.246960319621605 M17A-L47A 0.246504634667486 T50A-T105A 0.245682620756388 A259A-H305A 0.245548343530881 M19A-G200A 0.244538178861425 V167A-Y351A 0.243342435612245 M154A-W302A 0.24171673074515 G276A-H305A 0.240464468198443 M94A-V128A 0.240248373680918 G152A-W201A 0.23816998871369 F96A-G200A 0.232956026760785 H157A-W201A 0.232483231174434 L104A-A164A 0.232406018267273 P21A-L191A 0.232327069523567 T223A-T261A 0.231925559234707 G153A-A258A 0.231759742423924 I101A-V155A 0.231737641220131 I158A-V314A 0.231471793203475 P21A-L104A 0.230841404275551 G231A-I249A 0.230208240700313 A166A-L357A 0.229514577092174 S160A-S313A 0.226257149751951 T50A-L123A 0.22498397299613 G161A-D300A 0.224809346524434 W311A-T348A 0.22472374288849 S160A-S347A 0.224611702653766 S160A-A352A 0.224313655666471 T105A-W124A 0.22410037938075 G153A-A255A 0.22404729332361 V128A-V355A 0.222014083520877 G58A-L125A 0.221552962432296 G231A-V278A 0.221524780345883 T100A-T261A 0.220105294755012 S226A-I260A 0.220040830959222 A279A-Y284A 0.219444002320287 A272A-S313A 0.219109184432614 T105A-H305A 0.217671392838543 Y129A-V155A 0.217309341553417 W234A-S251A 0.216376085936257 A286A-I304A 0.216079626253707 V16A-V99A 0.215219313301916 P262A-T348A 0.213846664939952 S43A-G193A 0.213602403090556 Y57A-A122A 0.213375048879629 W198A-G231A 0.213161418328776 M19A-V156A 0.211696181080987 M19A-V99A 0.209918421044283 V20A-L196A 0.208585025869266 C283A-G312A 0.20755710803674 V155A-S347A 0.207236255875259 V23A-V99A 0.20701005588321 A166A-F353A 0.206331933033719 G22A-T105A 0.205424785200339 T100A-S159A 0.205289879266199 A98A-S159A 0.204147829216936 S159A-F353A 0.203230104714347 T126A-I358A 0.201890818217405 W311A-T349A 0.201844907069562 P262A-H305A 0.201572807023487 M19A-F96A 0.201315753153759 G200A-T261A 0.201284429983895 A253A-Y284A 0.201221656318449 A163A-L354A 0.200539830713398 S106A-L165A 0.199373508427733 A352A-L357A 0.19910362664988 V128A-F162A 0.198729962460022 V51A-I101A 0.198210026847419 N224A-G252A 0.197776449484137 G22A-A194A 0.196991974368585 I48A-V99A 0.196804048359422 F96A-G153A 0.196229523044705 A230A-I254A 0.196012461802777 A258A-I304A 0.195803517239039 V23A-H305A 0.19484605096332 F132A-V345A 0.19462721032855 T261A-I273A 0.193428980367029 M154A-T349A 0.192923032691149 L165A-T349A 0.192590053275065 I254A-G280A 0.192218651678187 F132A-M154A 0.191908539996636 T100A-T356A 0.191375091246758 W201A-N224A 0.191254323110304 I249A-C283A 0.190829151396798 F199A-A227A 0.189981491877302 V99A-S159A 0.189816663635052 V128A-H157A 0.189429608530185 T50A-I101A 0.188754489432164 G24A-A102A 0.188454088215488 A227A-A255A 0.188024248591208 F127A-V355A 0.187737304341955 I49A-A122A 0.187559209487274 S43A-M94A 0.187026387551865 A228A-S251A 0.186981100823835 M19A-L104A 0.186819730066141 A279A-S313A 0.186702580894897 T105A-A360A 0.186417529239953 W198A-G252A 0.186293182148729 T105A-I304A 0.186022220867321 T105A-W302A 0.185861297941182 H305A-L310A 0.184975800372348 A130A-S160A 0.184712479513685 M95A-F204A 0.184591179440337 M94A-S121A 0.184011546951767 G153A-G203A 0.18376350079809 H157A-Y351A 0.183618959847902 V233A-V278A 0.183609258732897 V51A-M94A 0.183386319444348 L18A-L197A 0.183352354280665 T50A-A97A 0.183200019761561 L257A-C283A 0.183122135643987 A303A-T349A 0.182778579069185 G58A-W124A 0.18262324514097 W54A-Y129A 0.182585248193077 M95A-S160A 0.180933192280233 G58A-T126A 0.180119116123296 T105A-T356A 0.180081662786842 F199A-F204A 0.179528990973305 G280A-L310A 0.179478703134125 M17A-G193A 0.179343431579503 L191A-G252A 0.179101420319839 A259A-I273A 0.179000110869876 M235A-G276A 0.178484797827137 G276A-L310A 0.178401158765649 G203A-N224A 0.178346445967769 S121A-L357A 0.178320704133613 I48A-A102A 0.177405842494961 T100A-W302A 0.176732006346494 L120A-S160A 0.176264296045406 G153A-P262A 0.175060604308769 A255A-G276A 0.174889105060367 G252A-I304A 0.174622870817041 V229A-W234A 0.17400659663254 T126A-T348A 0.172541395225071 V16A-V51A 0.172413825962542 W198A-T261A 0.172376669402717 V282A-V314A 0.170764746618003 A194A-I254A 0.170521006557776 A130A-A352A 0.170383960230545 H157A-D300A 0.170337581597375 F44A-T105A 0.170264148767787 S251A-A286A 0.170022785415838 A259A-I307A 0.169609039163921 S251A-L285A 0.168850676540914 W302A-T349A 0.168733900097283 T100A-T349A 0.167548978379415 A228A-I275A 0.167472224264948 F202A-A227A 0.167153053553782 F26A-T105A 0.167118785290006 V229A-V282A 0.167058027181853 G22A-A253A 0.16700780662319 G24A-A97A 0.166898896356969 G193A-I254A 0.166435200371573 Y284A-G309A 0.16611762902073 S159A-P262A 0.165646484833528 A350A-V355A 0.16560880772186 G152A-A163A 0.16550278889586 G193A-A255A 0.165316655455122 F26A-A301A 0.164752066245601 L104A-S121A 0.164514663143011 A130A-G153A 0.164410746459709 L123A-P131A 0.164290338049472 A228A-G276A 0.164168741399604 G161A-L357A 0.164117055035008 G203A-A227A 0.16403070073965 L197A-G252A 0.163980618100116 T225A-G252A 0.163793347786074 A97A-F204A 0.163607527944981 F56A-T126A 0.162687153303168 G153A-G256A 0.162651206961693 L285A-G308A 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