Welcome to PATHOS webserver

Protein pathogenicity prediction


PATHOS (PATHOgenicity Scoring) is a deep learning-based tool designed to predict the pathogenicity of protein variants directly from sequence data. The tool analyzes mutations and predicts their pathogenic potential using advanced machine learning models. PATHOS provides pathogenicity scores with a threshold of 0.63, where scores above this threshold indicate likely pathogenic variants. The tool processes multiple variants simultaneously, providing reliable pathogenicity assessments for clinical and research applications.

Database Coverage
Our database contains precomputed predictions for ~17,734 proteins from the human proteome, with over ~140 million mutations from SwissProt. For proteins not (yet) in our database or large batch jobs, use the standalone PATHOS package on GitHub to run predictions locally.

If you use PATHOS, please cite:

Radjasandirane R., Cretin G., Diharce J., de Brevern A.G., & Gelly J.C. (2026). PATHOS: Predicting Variant Pathogenicity by Combining Protein Language Models and Biological Features. Artificial Intelligence in the Life Sciences, 100165. doi:10.1016/j.ailsci.2026.100165

Prediction Input
Input UniProt IDs
Limits: Max 50 proteins per job · Max 10 full-landscape proteins · Max 1,000 mutations per protein · Max 100,000 total mutations · File upload max 1 MB.
Or, upload a TXT, CSV, or TSV file:
Format: first column is the UniProt ID, remaining columns or whitespace-separated values are mutations.

Job options
Webserver Statistics
Total Jobs
Unique Users
Jobs (Last 7 Days)
Mutations Analyzed
Activity (Last 30 Days)
Geographic Distribution