Enter one UniProt ID per line, optionally followed by specific mutations.
Specific mutations: Add mutations after the UniProt ID, separated by spaces.
Mutation format: Original_Residue + Position + New_Residue (e.g., M1R means Methionine at
position 1 changed to Arginine).
Full landscape: Enter a UniProt ID alone (no mutations) to view the complete
pathogenicity landscape with all possible mutations for that protein.
Mixed input example:
P04637 R158S P151S
N239K
P51787 P343A W305R Y545F
Q92521
P06401 L651V C347S
In this example, P04637 (without mutations) will show the full pathogenicity landscape, while
the other proteins will show predictions for their specified mutations.
File Upload:
Alternatively, you can upload a text file (.txt) containing the same format. Maximum file size:
1MB.
PATHOS will predict pathogenicity scores with a threshold of 0.63, where scores above this
threshold indicate likely pathogenic variants.
Usage Limits:
- Up to 50 proteins per job
- Up to 10 full-landscape proteins per job (lines with only a UniProt ID)
- Up to 1,000 mutations per protein
- Up to 300,000 total targeted mutations per job
- File upload: max 1 MB
- Rate limits: max 50 jobs per hour, 200 jobs per day,
with a 10-second cooldown between submissions